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The LDH release was determined using a LDH cytotoxicity detection kit (Roche, Mannheim, Germany) according to the manufacturers protocol

An important limitation of the present study is the substantial imbalance in case numbers across ancestry groups: European ancestry participants (2,302 cases) comprise the largest group, followed by African ancestry (1,339 cases), with considerably fewer cases among Admixed American/Latino participants (465 cases)

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The fluorescence intensity was recorded with continuous stirring every 30 sec for at least 5 min at 37 C in an FP-8500 spectrofluorometer (Jasco, Tokyo, Japan) with a fixed emission wavelength at 510 nm