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Alternative approaches to downregulate MYCNs activity in cancer target proteins involved in its oncogenic signalling pathways, such as bromodomain family members 20

Briefly, the lentiviral library containing the UPS-focused sgRNA library (Supplementary Data 2) was generated as described above and used to transduce KBM7 doxycyclin (DOX)-inducibleCas9 (iCas9) FKBP12-BFP-P2A-mCherry cells at a multiplicity of infection (MOI) of 0.1 and 1000x library representation