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Madine-Darby canine kidney (MDCK) epithelial cells were maintained in Eagles Minimum Essential Medium (EMEM) media (Sigma Aldrich) supplemented with 10% FBS, 5% penicillinstreptomycin and amphotericin B

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Using a transgenic fluorescent reporter approach, we also observed HIF-1 stabilization after OHT in RGCs and Mller glia, with the highest reporter tdTomato labeling appearing at 6 h and 3 days (Jassim et al., 2022)

Finally, in addition to murine models, in vivo drug screens are performed with transgenic Caenorhabditis elegans , Drosophila melanogaster , and zebrafish (please see reference for review of animal models used in ALS drug discovery [198])