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These findings establish a critical role for host glutathione in Rickettsia pathogenesis and contribute to an emerging paradigm that cytosol-dwelling bacterial pathogens sense the host reducing environment for specific aspects of virulence

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A 10 mg vial reconstituted with 1.0 mL of bacteriostatic water gives a 10 mg/mL concentration

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The cells were then washed with PBS buffer, and 1 10^6 cells were taken for each sample