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After reacting at 37 C for a given period of time under stirring at 400 rpm, the resulting solution was centrifuged to remove the degraded siRNAs (step 2)

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Cell Res 20:3450 Dutta S, Choudhury D, Roy S, Dattagupta JK, Biswas S (2016) Mutation in the pro-peptide region of a cysteine protease leads to altered activity and specificitya structural and biochemical approach