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Reverse Transcription-Quantitative Polymerase Chain Reaction The total RNA content was isolated from cells or the tumor using TRIzol reagent (Invitrogen), treated with DNase I (Thermo Fisher Scientific, Cat# EN0521), and then reverse transcribed into cDNA using a Maximal First Strand cDNA Synthesis Kit (Thermo Fisher Scientific, Cat# K1622)

Sienaert P, De Hert M, Houben M, Bouckaert F, Wyckaert S, Hagon B, Hagon A, Peuskens J

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BPC 157 also promoted the internalization of VEGFR2, a critical step for receptor activation

doi: 10.1055/s-0029-1220724