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Among cysteine modifications, S -thiolation is considered to be a reversible, non-enzymatic modification forming mixed disulfides with low molecular weight thiols 2

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However, genetic studies in yeast lacking both sam1/sam2 (MAT1/MAT2 orthologs) demonstrated that SeAM synthesis can occur but is highly toxic, which may explain the toxicity previously associated with its precursor SeMet [67]

Nakao et al