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Add each solvent one by one: 10% DMSO 90% (20% SBE--CD in Saline) Solubility: 2.08 mg/mL (7.19 mM)

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Absorbance of aqueous layer at 390 nm was measured with blank (5 mL of papain) ( For methionine estimation, 500 mg of sample was autoclaved with 6 mL of 2 N HCl for 1 h

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