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Other aspects of TCA cycle function in brain are completed around postnatal day 15, indicated by maximum oxidative response in rat brain slices to stimuli at this age (Holtzman et al., in vivo by day 10 in barrel cortex (Melzer et al., In the absence of pyruvate carboxylase activity during early postnatal development (Figure 9) glutamate synthesis within the brain must be replaced by import of glutamate or a precursor

10.1016/j.npep.2012.09.001 Neuropeptides 116 MarsdenJ

and 13000 g for 15 min, and the resulting supernatants processed using established protocols

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doi: 10.1074/jbc.274.20.13908 41 IslingerMLiKWSeitzJVlklALersGH