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Using expression of known marker genes, we annotated clusters corresponding to OL lineage cells (OPCs, committed oligodendrocyte precursors (COPs), and mature OLs (MOLs)), microglia, perivascular macrophages (PVMs), astrocytes, neural progenitor cells (NPCs), ependymal cells, endothelial cells, vascular cells, pericytes, T cells, and three clusters undergoing cell division (OPCs, microglia, and NPCs) (Fig

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The mixture was incubated at room temperature for 25 minutes and then added to the cells

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