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Isolation and quantification of RNA TRIzol reagent (16096020, Thermo Fisher Scientific, New York, USA) was used to extract total RNA

SIRT2 mediates NADH-induced increases in Nrf2, GCL, and glutathione by modulating Akt phosphorylation in PC12 cells

work showing that clock genes control G1/S progression by transcriptionally regulating Cdkn1a a gene encoding for the cyclin/Cdk inhibitor p12 WAF1/CIP1 and that alteration of circadian clock led to aberrant p21 expression and altered cellular proliferation 30