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A.LinC.GreenbergM

Among these key proteins, lipoylated proteins (particularly the PDH complex) and FeS cluster proteins serve as copper-binding sites and play critical roles in the cell (9)

doi: 10.1038/s41598-024-68966-8 135 EngelkeUFvan OutersterpREMerxJvan GeenenFAvan RooijABerdenGet al

After culture for 40 h, cells were collected and lysed in 8 ml of hypotonic buffer (10 mM HEPES pH 8.0, 10 mM KOAc, 1.5 mM Mg(OAc) 2 , 2% (v/v) glycerol, 5 mM NDSB-256 and 0.5 mM TCEP, with 1 tablet of cOmplete Mini EDTA-free Protease Inhibitor Cocktail (Roche, 11873580001) per 10 ml of buffer) by a Dounce homogenizer