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The filtered supernatant was run over a protein A/G column (Pierce), followed by extensive washing with PBS and the antibodies were eluted with 18 mL 0.1 M glycine pH 2.5 directly captured in 2 mL neutralization buffer (1 M TRIS pH 8.7)

Shin et al (159) reported that activation of the NRF2-ARE pathway contributes to the resistance of HNC cells to GPX4 inhibition, and that inhibition of this pathway reverses the resistance of HNC to ferroptosis

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