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This can occur due to exposure to UV light, which comes from sunlight and tanning beds

injection by ELISA

Certain children are more at risk for developing low vitamin D levels due to factors like ( skin color body weight sunlight exposure Children and teens who have higher levels of body fat are at higher risk of developing a vitamin D deficiency than kids who have standard body fat levels ( Additionally, kids with darker skin tones are significantly more likely to develop a vitamin D deficiency

Human immunodeficiency virus-restricted replication in astrocytes and the ability of gamma interferon to modulate this restriction are regulated by a downstream effector of the Wnt signaling pathway

and Rost, J., (1966) Analytical Biochemistry 15, 205-210