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Briefly, cells were rinsed with PM buffer (20 mM Na-phosphate, 1 mM MgSO 4 , pH 7.4) and fixed with 4% paraformaldehyde in PM buffer

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SA is directly bound to transforming growth factor beta-activated kinase 1 (TAK1), inhibiting NF-B and MAPK/ATF-2 pathways, thereby regulating mitogen-activated protein kinase (MLCK) expression

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