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Then, samples were centrifuged at 3660 g for 10 min and 150 L of the supernatant were transferred to a new microcentrifuge tube for dry vacuum

Depending on the experiment, a culture of 100 mL or of 2 L Luria-Bertani (LB) medium supplemented with 100 g/mL ampicillin (LB/Amp) was inoculated with 1/100 volume of an overnight culture and grown at 30 C to OD 600 0.4

In addition, the treatment of leukemic blasts with each of the two extracts combined with some drugs increases the sensitivity to death even in tumor cells that, in some cases, do not respond to conventional therapies

The best dietary sources of vitamin B12 are animal-based foods such as meat, fish, eggs, and dairy products [285, 286]

Scheibenbogen, Carmen (2020)