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A common target for peroxidation is unsaturated fatty acids present in membrane phospholipids

To testing ferroptosis inducers treatment, parental cells, persister cells, and regrown cells were treated with inducers alone or in combination with ferrostatin-1 (Fer-1)

Sharma R, Biedenharn KR, Fedor JM, Agarwal A

Buoyant fractions were collected using a tube slicer (Beckman-Coulter), additional fractions were pipetted from the top of the sucrose gradient in 1-ml increments and pellets were resuspended in 1 ml of HLM

Nonetheless, possessing supplementary biomarkers or surrogate measures (e.g., imaging, inflammatory markers, brain oedema, and atrophy), particularly in relation to specific therapeutic target engagement, can be highly beneficial for comparative analysis across species and in clinical trials