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Credit: Technology Networks A messenger RNA (mRNA) copied from DNA provides the instruction of which amino acid to incorporate at which position for the synthesis of a specific protein

Due to the very low expression of GRX1 in T cells, which does not upregulate upon activation, the GSH/GRX system cannot compensate for the loss of the TRX1 pathway, making TRX1 the sole electron donor for dNTP production in proliferating T cells

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The membrane was washed three times with TTBS for 5 min each and incubated with horseradish peroxidase conjugated goat anti-mouse IgG (Sigma) diluted (1:3000) in TTBS at 37C for 1 h