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The other half was fixed with 10% Formalin (Sigma) for 16 h at 4 C, transferred to a 70% v/v ethanol solution for a further 24 h and was then paraffin-embedded for immunohistochemistry

The lipid profiles for single drugs, synergistic and non-synergistic combinations will help further analysis and understanding of features of drug synergy

Functional complementary DNA clones were assembled and shown to be infectious by direct intrahepatic injection of RNA transcripts into chimpanzees 52,53