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A pre-incubation step in blot blocking buffer preceded the overnight incubation with primary antibodies against several biomarkers relevant to lung function and pathology, namely TGF-1 (1:1000, Abcam, UK), E-cadherin (1:1000, Bioss, China), Vimentin (1:1000, ABclonal, USA), MMP2 (1:1000, ABclonal), MMP9 (1:1000, ABclonal), COL1A1 (1:1000, cell signaling Technology, USA), Fibronectin (1:3000, Abcam), -Smooth Muscle Actin (1:1000, ABclonal), CTGF (1:1000, Abcam), SMAD2/3 (1:1000, ABclonal), and their phosphorylated counterparts, using GAPDH as a reference

The KD should reduce inflammation as it enhances various antioxidant mechanisms 14,15,16,17,18,19,20,21,22,23,24,25,26

In contrast, in underdeveloped countries with poor public health infrastructure, the incidence of EF is estimated to be approximately 100 cases per 100,000 people annually (Kim et al., 2022

HSP27 interacts with ACSL4 to modulate its stability, increasing ferroptosis-resistance in GBM cells [87]