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After transfection, the cells were plated in DMEM with 5% FBS and 400 mg/mL G418 for a month

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doi: 10.1038/s4159802063726w 264 AguilarETenaSempereMPinillaL

Furthermore, majority of the population in developing nations experience severe protein malnutrition, hunger and various illnesses as a result of insufficient food availability and poor food quality (Schonfeldt & Hall 2012)