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Considerations Slower onset: Because the peptide must traverse the nasal membranes and then enter general circulation (and eventually reach brain/central nervous system targets), the onset tends to be more gradual

Neurological Function Score (Modified Longa System) Neurological deficits were evaluated 1 hour after ET-1 injection using the modified Longa scoring system: 0: No observable neurological deficit (excluded from analysis) 1: Failure to fully extend the left forepaw 2: Consistent circling to the left 3: Falling or leaning to the left side when ambulating 4: No spontaneous movement (severe infarction) Animals scoring between 1 and 3 were considered to have moderate ischemic deficits and were included in further analyses

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Exposure to arsenic was determined from analyses of arsenic in drinking-water, performed by the state health laboratory between 1976 and 1997