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1 2 Journal of Neurochemistry

Free Radic Res 1997

The genetic association between the genes encoding methionine synthase and N5,10-methylenetetrahydrofolate reductase suggests a close relationship between these enzymes and the tetrahydrofolate metabolism (Figure 2) [14, 15, 16, 17]

416,417,418 Frataxin activates persulfide transfer, which is essential for the assembly of ironsulfur clusters (FeS) in the mitochondria and the activity of the mitochondrial respiratory chain complex and other mitochondrial enzymes

Immunostaining After organotypic culture or cryosection, the cochlear explants or tissue sections were fixed with 4% PFA, permeabilized with 1% TritonX-100 in PBS, and blocked by incubation in PBT-1 solution (0.1% Triton X-100, 8% donkey serum, 1% bovine serum albumin, and 0.02% sodium azide in PBS) at room temperature for 1 h