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Figure 7 These data also raised the possibility that the ADA assay was not detecting true ADA, but 1:1 complexes of endogenous anti-TNF antibodies and trimeric TNF, since partially bound TNF trimer could still theoretically bridge two molecules of AMG 966 in the antibody assay, and the etanercept spiked into the assay buffer to address this issue would not be able to neutralize partially bound TNF

van Dam RM, Hu FB, Willett WC