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The cells were transfected with complex of vector DNA (the FLAG-tagged N188Q ghrelin receptor (control) or the indicated FLAG-tagged ghrelin receptor mutants) and FuGENE HD transfection reagent (Promega)

Kors S, Hacker C, Bolton C, Maier R, Reimann L, Kitchener EJA, Warscheid B, Costello JL, Schrader M (2022) Regulating peroxisomeER contacts via the ACBD5-VAPB tether by FFAT motif phosphorylation and GSK3

Beyond general insomnia, some medications can trigger more specific sleep problems, including distressing nightmares or the uncomfortable sensations of Restless Legs Syndrome (RLS)