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The primary neuronal cells were extracted and cultured for 7 days, treated with MH or DMSO for 2 h, and then with ROT or DMSO for 24 h, to form a PD cell model, which was used for the experiments described below

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2.1 Physical and chemical synthesis Physical methods, such as evaporation-condensation, spark discharge, and pyrolysis, offer rapid synthesis without hazardous chemicals but suffer from high energy consumption, low yield, and inconsistent particle distribution (Kruis et al., 2000

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