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doi: 10.1038/s41586-019-1705-2 23 KraftVANBezjianCTPfeifferSRingelstetterLMllerCZandkarimiFet al

We performed an in vitro protein methylation assay using recombinant mouse AS3MT glutathione S-transferase (GST) fusion protein purified from bacteria and a radiolabeled S-adenosyl-L-methionine (SAM) [ 3 H] to visualize the methylation of potential substrates (Bedford et al