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Slides were stored at 80 C until immunostaining and imaging as described above

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To control that this basal activity is really mediated by MRP1, we mutated in MRP1 WT a cluster of 6 residues that were proven to be involved either in GSH binding or transport, R1138, R1142 38 , S1252, E1253 39 , or in binding of the GSH moiety of LTC 4 , R1249 and N1245 40

Bound T4 is attached to proteins that render it biologically inactive

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