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Basal HO-1 mRNA expression levels in response to injurious stimuli appear to be more important than the degree of upregulation or fold induction for the protection against those stimuli in the vasculature

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Needed for bone growth and bone remodeling by osteoblasts and osteoclasts Prevention of rickets in children and osteomalacia in adults Together with calcium, vitamin D also helps protect older adults from osteoporosis

Plasmid DNA was prepared using the Qiagen Plasmid Maxi Kit or GenElute HP Endotoxin-Free Maxiprep kit (Sigma) according to the manufacturers instructions