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After plasma membrane sheets were obtained and verified by microscopic observation, the ice-cold K-Glu buffer was replaced with room-temperature K-Glu buffer, and they were incubated with 2 M recombinant -SNAP WT or M105I for 5 min (PBS alone was used as negative control)

In BV2 microglial cell lines subjected to lipopolysaccharide (LPS)-induced neuroinflammation, both lactylation-mediated inhibition of p53 activity and direct knockdown of p53 protein can disrupt the control of the NF-B inflammatory signaling pathway, ultimately leading to abnormal expression of pro-inflammatory factors and M1 polarization of microglia [132]

Several hundred hemoglobinopathies caused by various mutations in globin genes are known, but only those associated with sickle cell disease are prevalent enough in the United States to merit discussion

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iii) reducing eNOS uncoupling by increasing tetrahydrobiopterin concentration and Hsp90/eNOS interaction