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The double-stranded DNA homology-directed repair (HDR) template (HiBiTBRD4 with extensions, shown below), the RNP complex and an electroporation enhancer (Integrated DNA Technologies) were then electroporated into Jurkat cells using an Amaxa 4D-Nucleofector (Lonza)

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In-Vitro Pharmacology and Selectivity Biochemical profiling places 5-Amino-1MQ potency for research NNMT in the low-micromolar IC range under standardized assay conditions, with cell-based EC values in the low micromolar range for lowering 1-MNA and suppressing lipogenesis