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SKU: 66878424478
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To purify FBU-His-EEHV-EE2, cell pellets were incubated in lysis buffer (20 mM Tris-HCl, pH 7.8, 0.5 M NaCl, 10 mM imidazole, 6 M guanidine hydrochloride (GdmCl)) for 15 min on ice

This damage was associated with significant lipid peroxidation, which could be mitigated by the antioxidants trolox, butylated hydroxytoluene, N-acetylcysteine, and membrane-permeable iron chelators such as 2,2-dipyridyl, 1,10-phenanthroline, LK614 LK616, and deferoxamine