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Tissue and serum samples were mixed with the R-A Buffer (Metallogenics) for 5 minutes, and the baseline absorbance (OD1) was determined

The top layer was aspirated without perturbing the disk, additional MeOH (400 l) was added, and the proteins were pelleted (10,000 g, 10 min, 4C) and the pellet were resuspended in 300 L of 50 mM TEAB, containing 2 M urea by water bath sonication

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Loss of the glycine N-ethyltransferase gene leads to steatosis and hepatocellular carcinoma in mice

doi: 10.4049/jimmunol.1301859 40 ShiLZWangRHuangGVogelPNealeGGreenDRet al