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However, it needs the right mix of nutrients to work well

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doi: 10.7150/jca.37335 179 WangYXieJWangHHuangHXieP

doi:10.1038/srep34248

The method according to any one of claims 7 to 11, characterized in that the calcium salt of N 5 , N 1o- methylene-5,6,7,8-tetrahydrofolic acid is prepared by reaction at room temperature of 5,6,7,8- Tetrahydrofolic acid in water with a compound selected from the group consisting of alkali metal hydroxides, alkali metal carbonates, alkali metal bicarbonates, ammonia and organic bases containing nitrogen, such as pyridine, triethylamine, triethanolamine, then a water-soluble, aromatic, nitrogen-containing base to this salt solution thus prepared adds which is selected in particular from the group consisting of pyridine and substituted pyridines, for example picolines, lutidines, ethylpyridines, pyridine being preferred, then reacting the salt solution thus prepared with formaldehyde, and then adding an aqueous solution of a calcium salt, where the calcium salt of N 5 , N 1 -methylene-5,6,7,8-tetrahydrofolic acid re fails