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In this chapter, we explain the widely used GST pull-down assay to identify the proteinprotein interaction between purified proteins

In terms of disease treatment, regulating lactylation levels in pathological states presents a potential strategy

To achieve early diagnosis and overall management of PD, key techniques and challenges include constructing a clinical database, bolstering PD early biomarker research, interpreting the clinical significance of biomarkers, enhancing interdisciplinary collaboration, establishing a joint diagnosis and AI diagnostic system using multiple biomarkers, as well as setting up a one-stop diagnostic and treatment center in partnership with community-based health service centers to screen, manage, and educate the high-risk PD populations