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Mudgal and coworkers demonstrated that in Swiss mice treated with LPS, a one-week treatment with a low dose of caffeine and a low dose of caffeic acid produced anxiolytic effects in the OFT and DLB, antidepressant effects in OFT and DLB, and anti-inflammatory effects with reduction of IL-6 and TNF-alpha in serum and brain [34]

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The cell precipitate was resuspended in Neurobasal medium (Gibco, 21103049) supplemented with 2% B-27 (Gibco, 17504044) and 0.5 mM l-glutamine, and the cells were seeded on plates pre-coated with poly-d-lysine hydrobromide (Sigma-Aldrich, P0296)

In addition, earlier studies have shown that the interaction of rutin with ascorbic acid leads to an increase in the reducing properties of ascorbic acid, which is expressed by a decrease in xanthine oxidase activity, responsible for the cytosol production of superoxide anion [74]