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for preparing a final formulation

Upon secretion, procollagen undergoes three steps of transformation in the extracellular space, i.e., (1) enzymatic removal of the free endings by procollagen peptidase to give rise to tropocollagen, (2) cross-linking of tropocollagen molecules to form collagen fibrils by lysyl oxidase, and (3) side-by-side cross-linking of collagen fibrils to form collagen fibers [54]

Blood (2014) 123(5):62531

Spiking experiments involved addition of known amounts of pure NAD and glutathione compounds individually at different stages of the extraction (Supplementary Table 1), followed by quantification using colorimetric assays

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