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We show that AsALG-4 26G-RNAs appear during the late stages of spermatogenesis commensurate with when their target mRNAs disappear

In the first step, glutamate and cysteine are ligated by -glutamylcysteine synthetase (also known as glutamatecysteine ligase, GCL) to form -glutamylcysteine, utilizing one molecule of ATP

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