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Solid YPD medium containing 200 g ml -1 hygromycin was used for selection of gsh2 deletion mutants, and medium containing 100 g ml -1 nourseothricin was used to select for the gsh2::GSH2 reconstituted strain in the gsh2 mutant background

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Cell 158 , 481491 (2014)

Likewise, while many evolutionary changes may be related to the generation of new genes, it is broadly recognized that the bulk of interspecies differences arise corollary to changes in gene activity