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NADH levels were measured in FoB from splenocytes, and were measured as mean autofluorescence intensity upon excitation with UV laser and recorded with a band pass filter BP465/30 133,134

doi:10.3746/pnf.2021.26.2.109

Alignments were masked and evolutionary trees created as described in detail in the methods

Oxidised glutathione (GSSG) is the "spent" form that needs to be converted back to GSH before it can function

During ischemia, ROS levels are modest, but when oxygen supply is restored during reperfusion, high levels of ROS and substantial damage to cardiac cells are produced