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Eide et al

The ovary, Malpighian tubules, salivary gland and midguts of the engorged females were dissected in phosphate buffer, frozen with liquid nitrogen and stored at -80 C, and three different developmental periods, including eggs, larvae and adults, were collected for subsequent assays

Davignon J, Gregg RE, Sing CF

They demonstrated the binding specificity between SAH-ubiquitin-conjugating enzyme E2 A (SAH-UBE2A) and E1 through a fluorescence microscopy-based qualitative binding assay and examined the specificity for disrupting the transfer of UBE1 thioester in the reconstituted system

Conclusion: Questionnaire-based referral from primary care followed by broadly available fast-track TE and eventually liver biopsy for selected patients is the standard practice in our unit