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Retaining the two outliers the same trend is observed with the modified MISI yielding a correlation of = 0.526 with the ssGIR versus = 0.469 for the standard method (n = 71)

Similarly, exposing a human cervical carcinoma cell line to aspartame at 10 mM resulted in significant cytotoxicity, measured by binding of sulforhodamine B to cellular proteins, binding of propidium iodide to DNA in necrotic cells, and measurement of intracellular ROS (DCFDA fluorescence) (Pandurangan et al., 2016a)

Biol Chem (2008) 389:52136.10.1515/BC.2008.064 14 KhanFPorterMSchwenkRDebotMSaudanPDuttaS

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Cleaved caspase-3 activity assay A cleaved caspase-3 ELISA kit (Cell Signaling Technologies, USA) was used to measure endogenous levels of cleaved caspase-3 protein in vivo and in vitro