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For immunohistochemistry (IHC), sections were deparaffinized and rehydrated through graded alcohols, followed by heat-induced antigen retrieval using citrate buffer (pH 6.0

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Vitamin E also showed no positive effect in a trial of patients with alcoholic cirrhosis who received supplements of the compound (de la Maza et al

4HNE binding requires the -loop, a large sequence motif that is inserted within the substrate recognition domain and is unique to GDAP1

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