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2179-A FIRST RESULTS OF THE EVOLUTION AND THE INFLUENCE OF STATIN TREATMENT ON NAFLD IN A PRIMARY CARE COHORT OVER A 2-YEAR PERIOD Leen Heyens 1,2,3 , Wouter Robaeys 2 , Liesbet Vernijns 4 , Anneleen Robaeys 5 , Sven Francque 6 and Geert Robaeys 2 , (1)Ziekenhuis Oost-Limburg, (2)Hasselt University, (3)Maastricht University, (4)Huisartsenbox, (5)Huisartsenpraktijk Termolen, (6)University of Antwerp, Edegem, Belgium Background: Non-alcoholic fatty liver disease (NAFLD) has become the most frequent cause of chronic liver disease

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The membrane was first blocked in 5% (wt./vol.) skim milk powder (Lab M Limited, Heywood, UK) in tris-buffered saline with 0.1% tween20 (TBST) for 1 h at RT, then was probed with the primary antibody (concentrations and manufacturers in Supplementary Table 4) diluted in TBST with 5% BSA for 16 h at 4 C on a rocker

However, as elucidated by Ecker [132], it is important to note that the neuroanatomical differences are neither unique to nor necessarily causal for ASD