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The levels of 4-HNE are remarkably elevated under conditions of oxidative stress and it is considered as one of the reliable biomarker of oxidative stress 64

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Arch Dermatol

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Activation of the Nrf2 pathway enhances the transcription of antioxidant response element (ARE)-dependent genes such as heme oxygenase-1 (HO-1), NAD(P)H quinone dehydrogenase 1 (NQO1), and glutamatecysteine ligase (GCL), thereby promoting detoxification of ROS and maintenance of redox homeostasis in oocytes (34)