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After natural cooling, the slides were placed in PBS (pH 7.4) and washed three times for 5 mins each

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As used herein, a "homologous recombinant animal" is a non-human animal, preferably a mammal, more preferably a mouse, in which an endogenous NOVX gene has been altered by homologous recombination between the endogenous gene and an exogenous DNA molecule introduced into a cell of the animal, e.g., an embryonic cell of the animal, prior to development of the animal

Testing oxidative stress markers such as MDA and glutathione levels can clarify whether your antioxidant system is compromised