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Hemolysis Assay To evaluate the hemocompatibility of MCNs/Ln/GD/FR NPs, rabbit blood was collected and centrifugated at 2000 g min 1 for 10 min at 4 C to isolate the red blood cells, which further washed and diluted with PBS solution by 10 times

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These modifications in metabolism pathways resulted in a substantial increase in the levels of 48 metabolites such as Xanthine, ascorbate, Cys-Gly, kynurenine, phenylalanine, 5-hydroxykynurenine, and others as outlined in Supplementary Figures 5A, B

Finally, vitamin B 12 status has been associated with NTD risk modification in the presence of specific polymorphisms in one-carbon metabolism (57)

Pasternak M., Lim B., Wirtz M.,Hell R., Cobbett C.S