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Hbv-induced n6 methyladenosine modification of parp1 enhanced afb1-related dna damage and synergistically contribute to hcc

10.3389/fimmu.2025.1454306 140

Additionally, the different concentrations of PDA@Pt solutions (0, 25, 50, 100 g/mL) were irradiated using an 808 nm laser (1 W/cm 2 , 10 min), with concurrent monitoring of temperature variations

Aber: 1

Quantitative assessment of the target genes expression levels was performed using real-time quantitative PCR (RQ-PCR) with a PikoReal Real-Time PCR System (Thermo Scientific) with iQ SYBR Green PCR Master Mix (Bio-Rad, Hercules, CA, USA), as described previously 39,40