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target enolase to achieve cellular specificity of glycolytic inhibition

BL has been shown to promote nuclear translocation of Nrf2, thereby upregulating HO-1 and enhancing the production of key antioxidant enzymes, including GSH, superoxide dismutase (SOD), and NAD(P)H quinone oxidoreductase 1 (NQO1) (Alural et al., 2015)

At the indicated times post-infection, coverslips were washed once with PBS and fixed in 4% paraformaldehyde (Ted Pella Inc., 18505, diluted in 1 x PBS) for 10 min at room temperature

Identifying these influences helps distinguish between medication failure and external variables affecting results