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The third mechanism involves decreased drug uptake due to changes in membrane permeability, caused by alterations in the characteristics of the porin channel

doi: 10.1016/j.biotechadv.2013.06.007, 78 NapoleoniM.KlennerF.KhawajaN.HillierJ

R., Genuardi, M., and Zammarchi, E

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Approximately 50 mg of frozen cecal content was added to 0.1 mm glass bead-containing tubes (PowerBead Tubes, Qiagen) prefilled with 300 l RLT buffer (RNeasy mini kit, Qiagen) supplemented with -mercaptoethanol (10 l/ml, Sigma-Aldrich) and 1 ml TRIzol (Invitrogen)